Part:BBa_K3782024:Design

cspA-RBS-TEE-His-DcAFP-Term

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 543
  Illegal BglII site found at 611
  Illegal BglII site found at 1088
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1097
  

Design Notes

The sequence was codon optimized for optimal expression in Escherichia coli.

Source

The source of DcAFP is Daucus carota. Its sequence was retrieved from GenBank. https://www.ncbi.nlm.nih.gov/nuccore/1139877302 The part was synthesized with a 6x His tag.